Bio-analytical Estimation of Losartan and Losartan Carboxylic acid Concentrations in Human Plasma by Using LC-MS/MS.

Authors

  • Ganesh N. Sharma School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, INDIA
  • Bhaswat S. Chakraborty Research & Development, Cadila Pharmaceuticals Limited, Dholka, Ahmedabad, Gujrat-387810, INDIA
  • Jyotsana Sanadya Dr. Shree RMS Institute of science and Technology, Bhanpura, M.P. INDIA; Jaipur College of Pharmacy, Sitapura, Jaipur, Rajasthan, INDIA
  • Uttam Singh Baghel School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, INDIA
  • Chinmoy Ghosh Research & Development, Cadila Pharmaceuticals Limited, Dholka, Ahmedabad, Gujrat-387810, INDIA
  • Manmohan Singhal School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, INDIA

Abstract

Losartan, is highly effective blood pressure lowering agent, which directly works by selectively blocking angiotensin-I (AT-I) receptor. It is primarily metabolized in active form, Losartan carboxylic acid (LCA). A sensitive and selective LC-MS/MS method for the determination of Losartan and Losartan Carboxylic Acid in human plasma was developed and validated. The ammonium formate: methanol (50:50 V/V) buffer was selected as the mobile phase; as the retention time was about only 1 minute and valsartan was taken as internal standard, due to resemble structural and chemical nature with the Losartan. The results were reproducible and precise with the samples prepared by solid phase extraction technique using “OASIS” HLB Cartridges (30mg), during method development trials; therefore it was used in sample preparation, for whole bioanalytical study of Losartan. The limit of quantification was from 25 ng/ml to 1500 ng/ml.

Keywords:

Bio-Analysis, Losartan, Losartan carboxylic acid, LCMS/MS

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Published

2025-01-01
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Vol. 2 No. 1 (2010): Volume 2,Issue1

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Articles