ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) : A CHROMATOGRAPHY TECHNIQUE
Abstract
UPLC can be regarded as a new direction for liquid chromatography. UPLC refers to Ultra Performance Liquid Chromatography.UPLC, improve in three areas: “speed, resolution, and sensitivity”. In this system uses fine particles (less than 2.5µm) so decreases the lengh of column, saves time and reduces solvent consumption.Keywords:
Ultra Performance Liquid Chromatography.References
1. Jerkovich A.D., Mellors J.S., and Jorgenson J.W., LCGC 21(7), 660-611 (2003).
2. Wu N. , Lippert J.A., and Lee M.L., J. Chromotogr. 911(1) (2001).
3. Unger K. K., Kumar D., Grun M., Buchel G., Ludtke S., Adam Th., Scumacher K., and Renker S., J. Chromatogr., A 892(47) (2000).
4. Swartz M. E. and Murphy B., Lab Plus Int., 18(6) (2004).
5. Swartz M. E. and Murphy B., Pharm. Formulation Quality 6(5), p. 40 (2004).
6. Van Deemter JJ, Zuiderweg EJ, Klinkenberg A.Longitudinal diffusion and resistance to mass transfer as causes of non ideality in chromatography. Chem. Eng. Sci. 1956; 5: 271-289
7. Zhang Y.H., Gong X.Y., Zhang H.M., Larock R.C., and Yeung E.S., J. Comb. Chem. 2, 450-452 (2000).
8. Zhou C., Jin Y., Kenseth J.R., Stella M., Wehmeyer K.R. and. Heineman W.R, J. Pharmac. Sci. 94, 576-589 (2005).
9. Zhu J., Goodall D.M., and Wren S.A.C., LCGC 23(1), 54-72 (2005).
10. Greibrokk T. and Andersen T., J. Chromatogr, A1000, 743-755 (2003).
11. Gerber F., Krummen M., Potgeter H., Roth A., Siffrin C., and Spoendlin C., J. Chromatogr., A1036, 127-133 (2004).
12. Tanaka N., Kobayashi H., Nakanishi K., Minakuchi H., and Ishizuka N., Anal. Chem. 73, 420A-429A (2001).
13. Wu N., Dempsey J., Yehl P.M., Dovletoglu A., Ellison A., and Wyvratt J., Anal. Chim. Acta 523, 149-156 (2004).
14. Swartz M. E. , Ultra Performance Liquid Chromatography (UPLC): An Introduction, Separation Science Re-Defined, LCGC Supplement, p. 8(MAY 2005)
15. Jerkovich A.D., Mellors J.S., and Jorgenson J.W., LCGC 21(7), 600-610 (2003).
16. MacNair J.E., Lewis K.C., and Jorgenson J.W., Anal. Chem. 69, 983-989 (1997).
17. Jerkovich A.D., Mellors J.S. and Jorgenson J.W., LCGC 21, 600-610 (2003).
18. Swartz M. E. Ultra Performance Liquid Chromatography (UPLC): An Introduction, Separation Science Re-Defined, LCGC Supplement, p. 10(2005)
19. MacNair J.E., Patel K.D. aqnd Jorgenson J.W., Anal. Chem. 71, 700-708 (1999)
20. Wu N., Lippert J.A. and Lee M.L., J. Chromatogr., A 911, 1-12 (2001).
21. Swartz M. E. , Ultra Performance Liquid Chromatography (UPLC): An Introduction, Separation Science Re-Defined, LCGC Supplement,p. 12(2005)
22. Lars Y. and Honore H.S., J. Chromatogr., A 1020, 59-67 (2003).
23. McLoughlin D.A., Olah T.V., and Gilbert J.D., J. Pharm. Biomed. Anal. 15, 1893–1901 (1997).
24. Swartz M. E. Ultra Performance Liquid Chromatography (UPLC): An Introduction, Separation Science Re-Defined LCGC Supplement, p. 11(2005)
25. Lippert J.A., Xin B., Wu N., and Lee M.L., J. Microcolumn Sep. 11, 631-643 (1997).
26. Swartz M. E., Ultra Performance Liquid Chromatography (UPLC): An Introduction, Separation Science Re-Defined LCGC Supplement, p. 13(2005)
27. Jerkovitch A.D., Mellors J.S., and Jorgenson J.W., LCGC 21(7), 2003.
28. Wu N., Lippert J.A., and Lee M.L., J. Chromotogr., 911(1), 2001.
29. Swartz M., LCGC 23(1), 46-53 (2005).
30. Broske A.D., et al., Agilent Technologies application note 5988-9251EN (2004).
31. Goodwin L, White SA, Spooner N. Evaluation of ultra-performance liquid chromatography in the bioanalysis of small molecule drug candidates in plasma. J. Chromatogr. Sci.; 45(6): 298-304, (2007).
Published
Abstract Display: 1 
PDF Downloads: 0 
