Development and Validation of HPTLC Method of Fluoxetine Hydrochloride in Bulk and Pharmaceutical Formulation

Authors

  • Zahid Zaheer Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, P.O.Box. No.33 Aurangabad-431001
  • Obaid Shaikh Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, P.O.Box. No.33 Aurangabad-431001
  • Sucheta Thorat Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, P.O.Box. No.33 Aurangabad-431001
  • Rana Ahmed Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, P.O.Box. No.33 Aurangabad-431001

Abstract

A simple, accurate, low cost and specific HPTLC method for estimation of Fluoxetine hydrochloride in capsule has been developed. It was performed on Silica gel G60 F254 aluminium foil using Acetone : Methanol in the ratio of 5:4 as mobile phase. The mobile phase containing chamber was saturated for 10 minutes at room temperature. The Rf value of Fluoxetine was found to be 0.12. The plate was scanned and quantified at 226 nm. The calibration curve response was observed between 300-2100 ng. The linear regression data showed good linear relationship of r² = 0.999. The percent recovery was found to be 99.94 ±1.188. The developed method was validated for its accuracy and precision with suitable parameters.

Keywords:

HPTLC, Fluoxetine Hydrochloride, Silica gel G60 F254

References

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Published

2025-01-01
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Issue

Vol. 2 No. 3 (2010): Volume 2,Issue3

Section

Articles